“Protein complexes are essential for proteins’ folding and biological function. Currently, native analysis of large multimeric protein complexes remains challenging. Structural biology techniques are time-consuming and often cannot monitor the proteins’ dynamics in solution. Here, a capillary electrophoresis-mass spectrometry (CE–MS) method is reported to characterize, under near-physiological conditions, the conformational rearrangements of ∽1 MDa GroEL upon complexation with binding partners involved in a protein folding cycle. … This study shows the CE–MS potential to provide information on binding stoichiometry and kinetics for various protein complexes.”

Find the paper and full list of authors in Advanced Science.