This book chapter (from “Salamanders: Methods and Protocols”) provides an alternative protocol to the in situ hybridization of amphibians. While this protocol “has been utilized for decades in axolotls, it has been challenging to implement consistently across tissues.” The authors here present an approach combining a hybridized chain reaction (HCR) with fluorescence in situ hybridization (FISH), providing a method with “a considerably higher signal to background” noise ratio.

See the full list of authors and read this book chapter, “Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue,” at the publisher’s page.